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mturquoise2 slbp aa18  (Addgene inc)


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    Addgene inc mturquoise2 slbp aa18
    (A) BJ-Fucci fibroblasts. BJ-5ta fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , <t>mTurquoise2-SLBP</t> <t>aa18-126</t> , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.
    Mturquoise2 Slbp Aa18, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mturquoise2+slbp+aa18/bio_rxiv__2025__09__12__675830-70-21-28?v=Addgene+inc
    Average 93 stars, based on 19 article reviews
    mturquoise2 slbp aa18 - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts"

    Article Title: Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts

    Journal: bioRxiv

    doi: 10.1101/2025.09.12.675830

    (A) BJ-Fucci fibroblasts. BJ-5ta fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , mTurquoise2-SLBP aa18-126 , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.
    Figure Legend Snippet: (A) BJ-Fucci fibroblasts. BJ-5ta fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , mTurquoise2-SLBP aa18-126 , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.

    Techniques Used: Transduction, Fluorescence, Imaging, RNA Sequencing, Expressing



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    mturquoise2 slbp aa18  (Addgene inc)
    93
    Addgene inc mturquoise2 slbp aa18
    (A) BJ-Fucci fibroblasts. BJ-5ta fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , <t>mTurquoise2-SLBP</t> <t>aa18-126</t> , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.
    Mturquoise2 Slbp Aa18, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mturquoise2+slbp+aa18/bio_rxiv__2025__09__12__675830-70-21-28?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    mturquoise2 slbp aa18 - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) BJ-Fucci fibroblasts. BJ-5ta fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , mTurquoise2-SLBP aa18-126 , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.

    Journal: bioRxiv

    Article Title: Cell-cycle dynamics of nascent transcription and mature RNA accumulation are concordant in normal fibroblasts

    doi: 10.1101/2025.09.12.675830

    Figure Lengend Snippet: (A) BJ-Fucci fibroblasts. BJ-5ta fibroblast cells are transduced with the Fucci4 cell-cycle reporters mKO2-Cdt1 aa30-120 , mTurquoise2-SLBP aa18-126 , Clover-Geminin aa1-110 , and mMaroon1-H1. (B) Nuclear fluorescence signals in a single BJ-Fucci cell (0–19 hr), daughter cell (19–43 hr), and grand-daughter cell (43hr–) measured by time-lapse imaging (duration 48 hr, interval 15 min). Circle, min-max normalized log 10 intensity. Line, Loess regression. Top, empirical cell cycle phase annotations. See for additional cells. (C) FACS of BJ-Fucci cells into early G1 (EG1), G1, early S (ES), and late S (LS)/G2/M phases. Populations indicated in the Clover/mKO2 plot (left) are shown in mTurquoise2 histogram (right). EG1 and ES are subsets of Clover/mKO2 populations defined by mTurquoise2 intensity. (D) Principal component analysis (PCA) of RNA-seq biological replicates by their mature RNA abundance (log 10 RNA-seq TPM) in all genes. (E) Mature RNA abundance (gene-level z-score of log 10 RNA-seq TPM) of select genes known to be dynamic in expression during the cell cycle. (F) K-means clustering of the union of differentially expressed genes by their mature RNA dynamics (gene-level z-score of log 10 RNA-seq TPM). “Icons” are within-cluster mean of log 10 TPM z-score and used in subsequent figures to graphically represent cluster dynamics. (G) Molecular Function and Cellular Component GO terms enriched in dynamic mature RNA clusters. Top 3 most enriched terms from each cluster are selected and their enrichment in each cluster was shown. (H) Mature RNA abundance dynamics (log 10 RNA-seq TPM z-score) of five representative genes in each cluster.

    Article Snippet: To introduce Fucci4 fluorescent markers, we produced lentiviruses carrying Clover-Geminin aa1-110 -IRES-mKO2-Cdt1 aa30-120 (gift from Michael Lin; Addgene ID83841; RRID:Addgene_83841) or mTurquoise2-SLBP aa18-126 -IRES-H1-mMaroon1 (gift from Michael Lin; Addgene ID83842; RRID:Addgene_83842), individually.

    Techniques: Transduction, Fluorescence, Imaging, RNA Sequencing, Expressing